Publications

Recent and selected publications listed below, by year

2023

Proinflammatory cytokines driving cardiotoxicity in Covid-19
Maria Colzani, Johannes Bargehr, Federica Mescia, Eleanor Williams, Vincent Knight-Schrijver, Jonathan Lee, Charlotte Summers, Irina Mohorianu, Kenneth G C Smith, Paul A Lyons, Sanjay Sinha
Cardiovasc Res .2023 Dec 2:cvad174. PMID: 38041432 DOI: 10.1093/cvr/cvad174

Epicardially secreted fibronectin drives cardiomyocyte maturation in 3D-engineered heart tissues
Lay Ping Ong, Johannes Bargehr, Vincent R Knight-Schrijver, Jonathan Lee, Maria Colzani, Semih Bayraktar, William G Bernard, Silvia Marchiano, Alessandro Bertero, Charles E Murry, Laure Gambardella, Sanjay Sinha
Stem Cell Reports 2023 Apr 11 PMID: 37001515 PMCID: PMC10147941 DOI: 10.1016/j.stemcr.2023.03.002

Inducible apelin receptor knockdown reduces differentiation efficiency and contractility of hESC-derived cardiomyocytes
Robyn G C Macrae, Maria T Colzani, Thomas L Williams, Semih Bayraktar, Rhoda E Kuc, Anna L Pullinger, William G Bernard, Emma L Robinson, Emma E Davenport, Janet J Maguire, Sanjay Sinha, Anthony P Davenport.
Cardiovasc Res 2023 Mar 31;119(2):587-598. doi: 10.1093 PMID: 36239923

A phenotypic screen of Marfan syndrome iPSC-derived vascular smooth muscle cells uncovers GSK3β as a new target
Hongorzul Davaapil, Madeline McNamara, Alessandra Granata, Robyn G C Macrae, Mei Hirano, Martina Fitzek, J A Aragon-Martin, Anne Child, David M Smith, Sanjay Sinha.
Stem Cell Reports 2023 Feb 14;18(2):555-569. DOI: 10.1016 PMID: 36669494 DOI: 10.1016

Densified collagen tubular grafts for human tissue replacement and disease modelling applications
Alexander W Justin, Federico Cammarata, Andrew A Guy, Silas R Estevez, Sebastian Burgess, Hongorzul Davaapil, Agavi Stavropoulou-Tatla, John Ong, Aishwarya G Jacob, Kourosh Saeb-Parsy, Sanjay Sinha, Athina E Markaki
Biomater Adv 2023 Feb;14 5:213245 PMID: 36549149

2022

A single-cell comparison of adult and fetal human epicardium defines the age-associated changes in epicardial activity
Vincent R Knight-Schrijver, Hongorzul Davaapil, Semih Bayraktar, Alexander D B Ross, Kazumasa Kanemaru, James Cranley, Monika Dabrowska, Minal Patel, Krzysztof Polanski, Xiaoling He, Ludovic Vallier, Sarah Teichmann, Laure Gambardella, Sanjay Sinha.
Nat Cardiovasc Res 2022 Dec 21;1(12):1215-1229. PMID: 36938497. DOI: 10.1038

The Histone Deacetylase 9 Stroke-Risk Variant Promotes Apoptosis and Inflammation in a Human iPSC-Derived Smooth Muscle Cells Model
Alessandra Granata, Ioannis Kasioulis, Felipe Serrano, James D Cooper, Matthew Traylor, Sanjay Sinha, Hugh S Markus
Front Cardiovasc Med 2022 Mar 30;9:849664. PMID: 35433850. DOI: 10.3389

2021

Differential expression in humans of the viral entry receptor ACE2 compared with the short deltaACE2 isoform lacking SARS-CoV-2 binding sites
Thomas L. Williams, Gregory Strachan, Robyn G. C. Macrae, Rhoda E. Kuc, Duuamene Nyimanu, Anna L. Paterson, Sanjay Sinha, Janet J. Maguire, Anthony P. Davenport.
Article number: 24336 (2021). Published 21 December 2021

Regenerative and non-regenerative transcriptional states of the human epicardium: from foetus to adult and back again
Vincent R. Knight-Schrijver, Hongorzul Davaapil, Alexander Ross, Xiaoling He, Ludovic Vallier, Laure Gambardella, Sanjay Sinha.
bioRxiv 2021.10.11.462541; 2021.10.11.462541

Unravelling Form and Function: Improved function of engineered cardiac tissue through extra-cellular anisotropy.
Jamie A. Cyr, Maria Colzani, Semih Bayraktar, Vera Graup, Richard Farndale, Sanjay Sinha, Serena M. Best, Ruth E. Cameron.
doi: https://doi.org/10.1101/2021.08.06.455438

Cardiovascular ACE2 receptor expression in patients undergoing heart transplantation.
Johannes Bargehr, Patrick Rericha, Alex Petchey, Maria Colzani, Georgia Moule, Marie Chet Malgapo, Doris Rassl, Jason Tarkin, Greg Mellor, Fotis Sampaziotis, Teresa Brevini, Laure Gambardella, Martin R Bennett , Sanjay Sinha.
PMID: 34390216 PMCID: PMC849722 DOI: 10.1002/ehf2.13528

Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection.
Williams TL, Colzani MT, Macrae RGC, Robinson EL, Bloor S, Greenwood EJD, Zhan JR, Strachan G, Kuc RE, Nyimanu D, Maguire JJ, Lehner PJ, Sinha S, Davenport AP.
Commun Biol. 2021 Jul 29;4(1):926. doi: 10.1038/s42003-021-02453-y.PMID: 34326460

Global proteomic analysis of extracellular matrix in mouse and human brain highlights relevance to cerebrovascular disease.
Pokhilko A, Brezzo G, Handunnetthi L, Heilig R, Lennon R, Smith C, Allan SM, Granata A, Sinha S, Wang T, Markus HS, Naba A, Fischer R, Van Agtmael T, Horsburgh K, Cader MZ.
J Cereb Blood Flow Metab. 2021 Sep;41(9):2423-2438. doi: 10.1177 PMID: 33730931

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver.
Sampaziotis F, Muraro D, Tysoe OC, Sawiak S, Beach TE, Godfrey EM, Upponi SS, Brevini T, Wesley BT, Garcia-Bernardo J, Mahbubani K, Canu G, Gieseck R 3rd, Berntsen NL, Mulcahy VL, Crick K, Fear C, Robinson S, Swift L, Gambardella L, Bargehr J, Ortmann D, Brown SE, Osnato A, Murphy MP, Corbett G, Gelson WTH, Mells GF, Humphreys P, Davies SE, Amin I, Gibbs P, Sinha S, Teichmann SA, Butler AJ, See TC, Melum E, Watson CJE, Saeb-Parsy K, Vallier L.
Science. 2021 Feb 19;371(6531):839-846. doi: 10.1126/science.aaz6964.PMID: 33602855

SARS-CoV-2 Infects Human Pluripotent Stem Cell-Derived Cardiomyocytes, Impairing Electrical and Mechanical Function.
Marchiano S, Hsiang TY, Khanna A, Higashi T, Whitmore LS, Bargehr J, Davaapil H, Chang J, Smith E, Ong LP, Colzani M, Reinecke H, Yang X, Pabon L, Sinha S, Najafian B, Sniadecki NJ, Bertero A, Gale M Jr, Murry CE.
Stem Cell Reports. 2021 Mar 9;16(3):478-492. doi: 10.1016/j.stemcr.2021.02.008. Epub 2021 Feb 13.PMID: 33657418.

2020

Modulating hESC-derived cardiomyocyte and endothelial cell function with triple-helical peptides for heart tissue engineering
Colzani M, Malcor JD, Hunter EJ, Bayraktar S, Polkinghorne M, Krieg T, Cameron R, Best S, Farndale RW, Sinha S.
Biomaterials. 2020 Dec 16;269:120612. doi: 10.1016/j.biomaterials.2020.120612.PMID: 33385684

Natural Biomaterials for Cardiac Tissue Engineering: A Highly Biocompatible Solution
Qasim A. Majid, Annabelle T. R. Fricker, David A. Gregory, Natalia Davidenko, Olivia Hernandez Cruz, Richard J. Jabbour, Thomas J. Owen, Pooja Basnett, Barbara Lukasiewicz, Molly Stevens, Serena Best, Ruth Cameron, Sanjay Sinha, Sian E. Harding and Ipsita Roy
Front. Cardiovasc. Med., 23 October 2020 | https://doi.org/10.3389/fcvm.2020.554597

Aortic “Disease-in-a-Dish”: Mechanistic Insights and Drug Development Using iPSC-Based Disease Modeling
Hongorzul Davaapil, Deeti K. Shetty and Sanjay Sinha
Front. Cell Dev. Biol., 28 October 2020 | https://doi.org/10.3389/fcell.2020.550504

Effects of fibrillin mutations on the behavior of heart muscle cells in Marfan syndrome
Aalders J, Léger L, Van der Meeren L, Van den Vreken N, Skirtach AG, Sinha S, De Backer J, van Hengel J.
Sci Rep. 2020 Oct 7;10(1):16756. doi: 10.1038/s41598-020-73802-w.PMID: 33028885

Collagen scaffolds functionalized with triple-helical peptides support 3D HUVEC culture
Jean-Daniel Malcor, Emma J Hunter, Natalia Davidenko, Daniel V Bax, Ruth Cameron, Serena Best, Sanjay Sinha, Richard W Farndale
Regenerative Biomaterials, rbaa025, https://doi.org/10.1093/rb/rbaa025 Published: 18 August 2020

Genomic profiling of human vascular cells identifies TWIST1 as a causal gene for common vascular diseases. Nurnberg ST, Guerraty MA, Wirka RC, Rao HS, Pjanic M, Norton S, Serrano F, Perisic L, Elwyn S, Pluta J, Zhao W, Testa S, Park Y, Nguyen T, Ko YA, Wang T, Hedin U, Sinha S, Barash Y, Brown CD, Quertermous T, Rader DJ. PLoS Genet. 2020 Jan 9;16(1):e1008538. doi: 10.1371/journal.pgen.1008538. eCollection 2020 Jan. PMID: 31917787

2019

BNC1 regulates cell heterogeneity in human pluripotent stem cell-derived epicardium. Gambardella L, McManus SA, Moignard V, Sebukhan D, Delaune A, Andrews S, Bernard WG, Morrison MA, Riley PR, Göttgens B, Gambardella Le Novère N, Sinha S. Development. 2019 Dec 13;146(24)

Cell cycle regulators control mesoderm specification in human pluripotent stem cells.
Yiangou L, Grandy RA, Osnato A, Ortmann D, Sinha S, Vallier L.J Biol Chem. 2019 Nov 22;294(47):17903-17914. doi: 10.1074/jbc.RA119.008251. Epub 2019 Sep 12. PMID: 31515269

Glycoproteomic Analysis of the Aortic Extracellular Matrix in Marfan Patients.
Yin 殷晓科 X, Wanga S, Fellows AL, Barallobre-Barreiro J, Lu R, Davaapil H, Franken R, Fava M, Baig F, Skroblin P, Xing Q, Koolbergen DR, Groenink M, Zwinderman AH, Balm R, de Vries CJM, Mulder BJM, Viner R, Jahangiri M, Reinhardt DP, Sinha S, de Waard V, Mayr M. Arterioscler Thromb Vasc Biol. 2019 Sep;39(9):1859-1873. doi: 10.1161/ATVBAHA.118.312175. Epub 2019 Jul 18. PMID: 31315432

Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancers.
Nakagaki-Silva EE, Gooding C, Llorian M, Jacob AG, Richards F, Buckroyd A, Sinha S, Smith CW. Elife. 2019 Jul 8;8:e46327. doi: 10.7554/eLife.46327. PMID: 31283468

Epicardial cells derived from human embryonic stem cells augment cardiomyocyte-driven heart regeneration.
Bargehr J, Ong LP, Colzani M, Davaapil H, Hofsteen P, Bhandari S, Gambardella L, Le Novère N, Iyer D, Sampaziotis F, Weinberger F, Bertero A, Leonard A, Bernard WG, Martinson A, Figg N, Regnier M, Bennett MR, Murry CE and Sinha S. Nat Biotechnol.2019 Aug;37(8):895-906.

Method to Synchronize Cell Cycle of Human Pluripotent Stem Cells without Affecting Their Fundamental Characteristics. Yiangou L, Grandy RA, Morell CM, Tomaz RA, Osnato A, Kadiwala J, Muraro D, Garcia-Bernardo J, Nakanoh S, Bernard WG, Ortmann D, McCarthy DJ, Simonic I, Sinha S, Vallier L. Stem Cell Reports. 2019 Jan 8;12(1):165-179. doi: 10.1016/j.stemcr.2018.11.020. Epub 2018 Dec 27. PMID: 30595546

A Novel Human Pluripotent Stem Cell-Derived Neural Crest Model of Treacher Collins Syndrome Shows Defects in Cell Death and Migration.
Serrano F, Bernard WG, Granata A, Iyer D, Steventon B, Kim M, Vallier L, Gambardella L, Sinha S. Stem Cells Dev. 2019 Jan 15;28(2):81-100.

2018

Coupling of a specific photoreactive triple-helical peptide to crosslinked collagen films restores binding and activation of DDR2 and VWF.
Malcor JD, Juskaite V, Gavriilidou D, Hunter EJ, Davidenko N, Hamaia S, Sinha S, Cameron RE, Best SM, Leitinger B, Farndale RW. Biomaterials. 2018 Nov;182:21-34.

Contributions of BMPR2 Mutations and Extrinsic Factors to Cellular Phenotypes of Pulmonary Arterial Hypertension Revealed by Induced Pluripotent Stem Cell Modeling.
Kiskin FN, Chang CH, Huang CJZ, Kwieder B, Cheung C, Dunmore BJ, Serrano F, Sinha S, Morrell NW, Rana AA. Am J Respir. Crit Care Med. 2018 Jul 15;198(2):271-275.

New models to study vascular mural cell embryonic origin: implications in vascular diseases.
Sinha S, Santoro MM. Cardiovasc Res. 2018 Mar 15;114(4):481-491.

2017

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids. Sampaziotis F, Justin AW, Tysoe OC, Sawiak S, Godfrey EM, Upponi SS, Gieseck RL 3rd, de Brito MC, Berntsen NL, Gómez-Vázquez MJ, Ortmann D, Yiangou L, Ross A, Bargehr J, Bertero A, Zonneveld MCF, Pedersen MT, Pawlowski M, Valestrand L, Madrigal P, Georgakopoulos N, Pirmadjid N, Skeldon GM, Casey J, Shu W, Materek PM, Snijders KE, Brown SE, Rimland CA, Simonic I, Davies SE, Jensen KB, Zilbauer M, Gelson WTH, Alexander GJ, Sinha S, Hannan NRF, Wynn TA, Karlsen TH, Melum E, Markaki AE, Saeb-Parsy K, Vallier L. Nat Med. 2017 Aug;23(8):954-963.

Exposure of Induced Pluripotent Stem Cell-Derived Vascular Endothelial and Smooth Muscle Cells in Coculture to Hemodynamics Induces Primary Vascular Cell-Like Phenotypes. Collado MS, Cole BK, Figler RA, Lawson M, Manka D, Simmers MB, Hoang S, Serrano F, Blackman BR, Sinha S, Wamhoff BR. Stem Cells Transl Med. 2017 Aug;6(8):1673-1683. Stem Cells Transl Med. 2017 Aug;6(8):1673-1683.

Human Stem Cell-Derived Endothelial-Hepatic Platform for Efficacy Testing of Vascular-Protective Metabolites from Nutraceuticals. Narmada BC, Goh YT, Li H, Sinha S, Yu H, Cheung C. Stem Cells Transl Med. 2017 Mar;6(3):851-863.

An iPSC-derived vascular model of Marfan syndrome identifies key mediators of smooth muscle cell death. Granata A, Serrano F, Bernard WG, McNamara M, Low L, Sastry P, Sinha S. Nat Genet. 2017 Jan;49(1):97-109.

2016

Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha S, Kotter M, Vallier L. Development. 2016 Dec 1;143(23):4405-4418.

Embryological Origin of Human Smooth Muscle Cells Influences Their Ability to Support Endothelial Network Formation. Bargehr J, Low L, Cheung C, Bernard WG, Iyer D, Bennett MR, Gambardella L, Sinha S. Stem Cells Transl Med. 2016 Jul;5(7):946-59.

Vascular Smooth Muscle Cells in Atherosclerosis. Bennett MR, Sinha S, Owens GK. Circ Res. 2016 Feb 19;118(4):692-702.

2015

Robust derivation of epicardium and its differentiated smooth muscle cell progeny from human pluripotent stem cells. Iyer D, Gambardella L, Bernard WG, Serrano F, Mascetti VL, Pedersen RA, Talasila A, Sinha S. Development. 2015 Apr 15;142(8):1528-41.

Myocardin regulates vascular smooth muscle cell inflammatory activation and disease. Ackers-Johnson M, Talasila A, Sage AP, Long X, Bot I, Morrell NW, Bennett MR, Miano JM, Sinha S. Arterioscler Thromb Vasc Biol. 2015 Apr;35(4):817-28

Temporal and embryonic lineage-dependent regulation of human vascular SMC development by NOTCH3. Granata A, Bernard WG, Zhao N, Mccafferty J, Lilly B, Sinha S. Stem Cells Dev. 2015 Apr 1;24(7):846-56.

2014

Directed differentiation of embryonic origin-specific vascular smooth muscle subtypes from human pluripotent stem cells. Cheung C, Bernardo AS, Pedersen RA, Sinha S. Nat Protoc. 2014 Apr;9(4):929-38.

Embryonic origins of human vascular smooth muscle cells: implications for in vitro modeling and clinical application. Sinha S, Iyer D, Granata A. Cell Mol Life Sci. 2014 Jun;71(12):2271-88.

2013

Myocardin Regulates Vascular Response to Injury Through miR-24/-29a and Platelet-Derived Growth Factor Receptor-β. Talasila A, Yu H, Ackers-Johnson M, Bot M, van Berkel T, Bennett MR, Bot I, Sinha S. Arterioscler Thromb Vasc Biol. 2013 Oct;33(10):2355-65.

Embryological-origin-dependent differences in homeobox expression in adult aorta: role in regional phenotypic variability and regulation of NF-κB activity. Trigueros-Motos L, González-Granado JM, Cheung C, Fernández P, Sánchez-Cabo F, Dopazo A, Sinha S, Andrés V. Arterioscler Thromb Vasc Biol. 2013 Jun;33(6):1248-56.

2012

Vascular disease in a dish: all the right ingredients? Sinha S. Circulation. 2012 Oct 2;126(14):1676-7.

Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility. Cheung C, Bernardo AS, Trotter MW, Pedersen RA, Sinha S. Nat Biotechnol. 2012 Jan 15;30(2):165-73.

Myocardin overexpression is sufficient for promoting the development of a mature smooth muscle cell-like phenotype from human embryonic stem cells. Raphel L, Talasila A, Cheung C, Sinha S. PLoS One. 2012;7(8):e44052.

2011

Human embryonic stem cell-derived vascular smooth muscle cells in therapeutic neovascularisation. Cheung C, Sinha S. J Mol Cell Cardiol. 2011 Nov;51(5):651-64.

Yiangou L, Grandy RA, Osnato A, Ortmann D, Sinha S, Vallier L.J Biol Chem. 2019 Nov 22;294(47):17903-17914. doi: 10.1074/jbc.RA119.008251. Epub 2019 Sep 12. PMID: 31515269